EXAMINATION OF GLUCOSES CONCENTRATION IN CHRONIC SEPSIS MODEL IN RATS

Abstract

We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and pure culture of bacteria, with the aim to monitor glucose concentration in sepsis. The trial was carried out on 104 male rats, Wistar strain, body mass 190 to 240 g. The rats were divided in four groups consisting of 28 animals, and a control group of 20 animals. The period of observations were 12, 24, 72 and 120 hours after surgical intervention. In the examined sepsis model, a significant hypoglycemic activity was noticed in early sepsis (12 and 24 hours), but in the group with mixed bacteria culture only after 72 hours. Hyperglycemia was registered in late sepsis (72 hours) in the group of rats where sepsis was caused by pure bacterial culture of Escherichia coli and Staphylococcus aureus, without statistic significance compared to the findings in the control group.