COMPARISON OF METHODS FOR DETECTION OF MICROSPORIDIA SPECIES OF THE GENUS NOSEMA IN HONEY BEES (APIS MELLIFERA)
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Keywords

Apis mellifera
Nosema sp.
microscopic examination
simplex- PCR
duplex-PCR

How to Cite

1.
Glavinić U, Stanković A, Stevanović J, Simeunović P, Aleksić N, Stanimirović Z. COMPARISON OF METHODS FOR DETECTION OF MICROSPORIDIA SPECIES OF THE GENUS NOSEMA IN HONEY BEES (APIS MELLIFERA). AVM [Internet]. 2013 Sep. 6 [cited 2024 Jun. 24];6(1):19-27. Available from: https://niv.ns.ac.rs/e-avm/index.php/e-avm/article/view/142

Abstract

Two microsporidia species of the Nosema genus cause nosemosis in the adult honeybee: N. apis and N. ceranae. For diagnostic purposes and the determination of infection level various microscopic and molecular biological methods are used. Th e aim of this research was to compare the reliability of the traditional microscopic assessment and two PCR techniques: simplex- and duplex-PCR. Honey bee samples were taken from 150 colonies. Microscopic examination, performed according to the recommendations of the OIE, revealed Nosema spores in 68.67% samples analysed, whilst with the simplex-PCR method all samples (100.0%) proved positive. On the other hand, duplex-PCR method used for the identifi cation of Nosema species resulted in 84.0% positive samples, all of which were N. ceranae. Our recommendation of the simplex-PCR method for the monitoring of honey bees in fi eld conditions is based on its higher reliability than the microscopic assessment in the detection of low-level infections, as well as its potential for the detection of vegetative Nosema sp. stages; thus the early detection and timely prevention of Nosema infection would be possible. Nosema species identifi cation is simplest and most cost-eff ective if performed with the duplex-PCR analysis. However, the simplex-PCR is more reliable, thus, it is suggested that samples that were negative when assessed with microscopy and duplex-PCR analysis undergo simplex-PCR.

https://doi.org/10.46784/e-avm.v6i1.142
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