Abstract
This study had for objective to investigate by molecular tools the intestinal Eimeria infection in slaughtered dromedaries (Camelus dromedarius) from two abattoirs in Southern Algeria. In all 89 paraffin-embedded intestinal samples were analysed, and the DNA was extracted with a commercial kit. Polymerase chain reaction (PCR) was done after optimising the method, and correlations between the DNA concentrations and PCR results were assessed. Of the 85 DNA extracts tested, 12 (14.1%) were PCR-positive for Eimeria. Higher infection rates were observed in Sahraoui camels compared with the Targui breed, and in older animals compared with adults. A strong positive correlation was observed between DNA concentration and the success of PCR amplification. This work provides one of the first molecular confirmations of Eimeria infection in Algerian dromedaries. Further research is needed to identify Eimeria species and construct a phylogenetic tree to support epidemiological control strategies.
References
Abbas, I.E., El-Alfy, E., Al-Araby, M., Al-Kappany, Y., El-Seadawy, R., Dubey, J.P. 2019. Prevalence of Eimeria species in camels (Camelus dromedarius) from Egypt and variability in structure of Eimeria cameli oocysts. Journal of Parasitology, 105, 3, 395-400.
Ahmed, M.E., Hassan, O.A., Khalifa, A.K.A, Elobied, E., Osman, A.A.A., Brair, S.L., Osama, I.E.A., Elfadul, M.M.A., Cremers, A.L., Grobusch, M.P. 2018. Echinococcosis in Tambool, Central Sudan: A knowledge, attitude and practice (KAP) study. International Health, 10, 6, 490-494. https://doi.org/10.1093/inthealth/ihy055
Ahmed, M., Bakr, L., Abdelhamid, M. 2025. Parasitological and histopathological findings of naturally occurring coccidiosis in slaughtered camels (Camelus dromedarius) in Aswan Governorate, Egypt. Parasitologists United Journal, 18, 2, 155-159. https://doi.org/10.21608/puj.2025.390797.1299
Al-Afaleq, A.I., Elamin, E.A., Fatani, A., Homeida, A.G.M. 2018. Parasitic profile of Saudi Arabian camels. Journal of Camel Practice and Research, 25, 1, 93-97. https://doi.org/10.5958/2277-8934.2018.00014.0
Boukert, R., Saidj, D., Bekki, M.Y., Sahraoui, N. 2023. Prospective study on camel breeding in the province of Bechar. Revue des BioRessources, 13, 2, 100–111. Available at: https://journals.univ-ouargla.dz/index.php/RBR/article/view/2157 Accessed: 05.2025.
Boukert, R., Chikhaoui, M., Saidj, D., Babelhadj, B., Damene, H., Benali, S., Abdul Hussain, A.S., Sahraoui, N. 2024. Histopathological investigation and risk factors of coccidiosis in camels (Camelus dromedarius), Algeria. Emirates Journal of Food and Agriculture, 36, 1–11. https://doi.org/10.3897/ejfa.2024.118325
Bouragba, M., Laatamna, A., Cheddad, F.E., Baroudi, D., Houali, K., Hakem, A. 2020. Gastrointestinal parasites of dromedary camel (Camelus dromedarius) in Algeria. Veterinary World, 13, 8, 1635-1640. https://doi.org/10.14202/vetworld.2020.1635-1640
Bussière, F., Niepceron, A., Sausset, A., Esnault, E., Silvestre, A., Walker, R.A., Smith, N.C., Quéré, P., Laurent, F. 2018. Establishment of an in vitro chicken epithelial cell line model to investigate Eimeria tenella gamete development. Parasites and Vectors, 11, 1, 44. https://doi.org/10.1186/s13071-018-2622-1
Chartier, C., Paraud, C. 2012. Coccidiosis due to Eimeria in sheep and goats, a review. Small Ruminant Research, 103, 1, 84–92. https://doi.org/10.1016/j.smallrumres.2011.10.022
Craig, B.H., Pilkington, J.G., Kruuk, L.E., Pemberton, J.M. 2007. Epidemiology of Parasitic Protozoan Infections in Soay Sheep (Ovis aries L.) on St Kilda. Parasitology, 134, Pt1, 9–21. https://doi.org/10.1017/S0031182006001144
Djerbouh, A, Lafri, I, Kechemir-Issad, N, Bitam, I. 2018. Endo- and ectoparasites (Ixodidae) of camels (Camelus dromedarius) from Southern Algeria. Livestock Research for Rural Development 30, 8. Available at: http://www.lrrd.org/lrrd30/8/djerb30141.html Accessed: 05.2025.
Dubey, J.P., Schuster, A. 2018. A review of coccidiosis in Old World camels. Veterinary Parasitolgy, 262, 75-83. https://doi.org/10.1016/j.vetpar.2018.08.008
Ekawasti, F., Nurcahyo, R.W., Wardhana, A.H., Shibahara, T., Tokoro, M., Sasai, K., Matsubayashi, M. 2019. Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia. Parasitology International, 72, 101927. https://doi.org/10.1016/j.parint.2019.101927
Ekawasti, F., Nurcahyo, R.W., Nashrulloh, M.F., Priyowidodo, D., Prastowo, J. 2022. Development of a multiplex polymerase chain reaction technique for detection and discrimination of Eimeria spp. in cattle in Indonesia. Veterinary World. 15, 4, 975-980. https://doi.org/10.14202/vetworld.2022.975-980
Faye, B., Konuspayeva, G., Magnan, C. 2023. Large camel farming: A care-management guide from breeding to camel products, Springer Dordrecht. https://doi.org/10.1007/978-94-024-2237-5
Hassan, S.E., Toaleb, N.I., Shaapan, R.M., Abdel-Rahman, E.H., Elmahallawy, E.K. 2016. Diagnosis of toxoplasmosis using affinity-purified fraction of tachyzoites local isolate. Research Journal of Parasitology, 11, 1-2, 13-19. Available at: https://scialert.net/abstract/?doi=jp.2016.13.19 Accessed: 05.2025.
Hastutiek, P., Lastuti, N.D.R., Suwanti, L.T., Sunarso, A., Suprihati, E., Kurniawati, D.A., Matsubayashi, M. 2022. Coproparasitological examinations and molecular determination of Eimeria species in Madura cattle reared on Madura Island, Indonesia. Parasitology International, 86, 102478. https://doi.org/ 10.1016/j.parint.2021.102478
Hussein, H.S., Kasim, A.A., Shawa, Y.R. 1987. The prevalence and pathology of Eimeria infections in camels in Saudi Arabia. Journal of Comparative Pathology, 97, 3, 293-297. https://doi.org/10.1016/0021-9975(87)90093-4
Kawahara, F., Zhang, G., Mingala, C.N., Tamura, Y., Koiwa, M., Onuma, M., Nunoya, T. 2010. Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites. Veterinary Parasitology, 174, 1-2, 49-57. https://doi.org/10.1016/j.vetpar.2010.08.001
Kramer M.F., Coen D.M. 2001. Enzymatic amplification of DNA by PCR: standard procedures and optimization. Current Protocols in Molecular Biology. Chapter 15: Unit 15.1. https://doi.org/10.1002/047114 3030.cba03fs10
Malek, S.S., Kuraa, H.M. 2018. Detection and identification of Eimeria species in naturally infected calves at Assiut governorate. Zagazig Veterinary Journal, 46, 1, 60-69. https://doi.org/10.21608/zvjz.2018.7624
Mahmmod, Y.S., El-Balkemy, F.A., Yuan, Z.G,. El-Mekkawy, M.F., Monazie, A.M., Zhu, X.Q. 2010. Field evaluation of PCR assays for the diagnosis of tropical theileriosis in cattle and water buffaloes in Egypt. Asian Journal of Animal and Veterinary Advances, 9, 4, 696-699. https://doi.org/10.3923/javaa.2010.696.699
Maxamhud, S., Reghaissia, N., Laatamna, A., Samari, H., Remdani, N., Gentekaki, E., Tsaousis, A.D. 2023. Molecular identification of Cryptosporidium spp., and Giardia duodenalis in dromedary camels (Camelus dromedarius) from the Algerian Sahara. Parasitologia, 3, 2, 151-159. https://doi.org/10.3390/parasitologia3020016
Metwally, D.M., Al-Otaibi, T.T., Albasyouni, S.A., El-Khadragy, M.F., Alajmi, R.A. 2020. Prevalence of eimeriosis in the one-humped camels (Camelus dromedarius) from Riyadh and Al-Qassim, Saudi Arabia. PeerJ, 8, e10347. https://doi.org/10.7717/peerj.10347
Narnaware, S.D., Jyotsana, B., Ranjan, R., Dahiya, S.S, Prakash, V. 2024. The first molecular characterization of Sarcocystis cameli in the Indian dromedary camels (Camelus dromedarius). Veterinary Research Communications, 48, 3, 1929–1933. https://doi.org/10.1007/s11259-024-10348-6
Pikor, L.A., Enfield, K.S.S., Cameron, H., Lam, W.L. 2011. Extraction d'ADN de matériel inclus en paraffine pour les analyses génétiques et épigénétiques. Journal of Visualized Experiments, 26, 2763. https://doi.org/10.3791/2763
Qablan, M.A., Obornik, M., Petrzelkova, K.J. 2013. Infections by Babesia caballi and Theileria equi in Jordanian equids, Epidemiology and genetic diversity. Parasitology, 140, 9, 1096-1103. https://doi.org/10.1017/S0031182013000486
Raj, G.D., Aarthi, S., Selvabharathi, R., Raman, M., Blake, D.P., Tomley, F.M. 2013. Real-time PCR-based quantification of Eimeria genomes: A method to outweigh underestimation of genome numbers due to PCR inhibition. Avian Pathology, 42, 4, 304–308. https://doi.org/10.1080/03079457.2013.790531
Salama, A., Noaman, E.A., Nayel, M., El-kattan, A.M., Mona, A., Mahmoud, A.S, Abd El-hamid, D.I.S., Elsify, A., Zaghawa, A., Arbaga, A.A., Mousa, W. 2023. Prevalence and molecular characterization of four enteric protozoa in dromedary camels (Camelus dromedarius). Alexandria Journal of Veterinary Sciences, 78, 1, 17-27. https://doi.org/10.5455/ajvs.142867
Saidi, R., Mimoune, N., Chaibi, R., Abdelouahed, K., Khelef, D., Kaidi, R. 2022. Camel gastrointestinal parasites in southern Algeria. Veterinarska stanica, 53, 3, 283–294. https://doi.org/10.46419/vs.53.3.7
Sazmand, A., Hamidinejat, H., Hekmatimoghaddam, S., Asadollahi, Z., Mirabdollahi, S. 2012. Eimeria infection in camels (Camelus dromedarius) in Yazd province, central Iran. Tropical Biomedicine, 29, 1, 77-80.
Tafti, A.K., Maleki, M., Oryan, A., Mozafari, A.A. 2000. Pathological study of digestive system lesions of camels (Camelus dromedarius) slaughtered in Iran. In: Proceedings of 18th meeting of the European Society of Veterinary Pathology: Amsterdam, The Netherlands, 19-22 September, 245–5.
Toaleb, N.I., Raafat, M.S.M. 2024. Zoonotic protozoan parasites infecting camels, Diagnosis and control – A Review. Egyptian Journal of Veterinary Sciences, 55, 3, 1131-1142. https://doi.org/10.21608/EJVS.2023.25160 9.1686
Utebaeva, G., Berkinbay, O., Symbat Suttibaevna, U., Tuganbay, A. 2021. Study of prevalence and associated risk factors of Eimeria spp. in Camels in Turkestan Region. Archives of Razi Institute Journal, 76, 5, 1419-1425. https://doi.org/10.22092/ari.2021.355660.1707.
Verweij, J.J., Blangé, R.A., Templeton, K., Schinkel, J., Brienen, E.A., van Rooyen, M.A., van Lieshout, L., Polderman, A.M. 2004. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. Journal of Clinical Microbiology, 42, 3, 1220–1223. https://doi.org/10.1128/JCM.42.3.1220-1223.2004
Wei, J.G., Wang, Z.A. 1990. Survey of the Eimeria species in the double-humped camels of the Inner Mongolian Autonomous region. Chinese Journal of Veterinary Medicine, 16, 23–24.
Yakhchalim, M., Cheraghi, E. 2007. Eimeriosis in bactrian and dromedary camels in the Miandoab region, Iran. Acta veterinaria, 57, 5-6, 545-552. https://doi.org/10.2298/AVB0706545Y

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2026 Razika BOUKERT, Dahia Saidj, Sara BOUKERT, Baaissa Babelhadj, Mira Chikhaoui, Amir Abderraouf Benotmane, Başak Şentürk, Eylul Ece Islek Camadan, Buse Kevser Karadeniz, Gizem Sele, Tanil Kocagoz, Naima Sahraoui